Articles, announcements and insights from Horizon Discovery

Probing DNA Damage Response Pathways

Mar 8, 2017 1:02:47 PM No Comments

Sensitivity and resistance to DNA Damage Response Pathways identified with gene-edited cell lines and wildtype controls

The cellular DNA damage response (DDR) is an essential safeguard against cancer. Upon activation, the DDR can limit tumour progression at the early stages by inducing senescence or cell death. When this defence fails tumors are able to develop. However, with time, tumors accumulate more mutations in DNA repair proteins as cancers progress.  The efficiency of DDR plays an essential role in the effectivity of cytotoxic treatments. Currently much research is focussed on identifying the DDR mechanisms involved in cancers and how these dysfunctional processes can be utilized against tumor growth.

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How to Cure Cancer in 3 Easy Steps

Mar 6, 2017 6:10:15 PM No Comments

When I have read articles just like this one early on in my career, I would laugh and categorize it with blogs regarding Bigfoot and the Loch Ness Monster. However, much has changed in the past 10 years. New technologies have been developed and milestones have been reached that should have Cancer a little worried. These 3 steps might be viewed to some as obvious, but I argue that it’s how the researchers have utilized the technology wisely that has made the difference. I have identified some papers that have carved a successful path to Cancer's possible demise.

Read More Cell lines, Gene editing, CRISPR, Oncology Panels, Target identification, Target validation, PDX, Cancer models, Preclinical efficay testing, High Fidelity Models

Spying on proteins - How observation can affect the perception of reality

Mar 2, 2017 2:47:38 PM No Comments

One of the highest ideals in science is to observe natural events in their native context. Doing so is a constant challenge thanks to the Observer Effect, described by Heisenberg and others, where the act of observing or measuring a process alters it. Thus, scientists of all stripes try to get out of the way, attempting to produce the most accurate measurements possible using specific yet unobtrusive tools. Wildlife photographers use long-range lenses to avoid the need to stand directly in front of a herd of water buffalo and thereby affect the animals’ behaviour. Psychologists create tests where the subjects are unaware of the true intent so as to minimize changes in natural responses.

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Large scale gene tagging simplified by a self releasing plasmid

Mar 2, 2017 2:19:09 PM No Comments

Knockout and tagged gene-editing can create cell lines ideal for antibody validation. Using CRISPR CAS technology, Horizon developed a streamlined process to cut production time. Read more in our interview with Lead Scientist Daniel Lackner

Audio also available

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Endogenously tagged reporter cell lines to improve antibody validation

Mar 1, 2017 8:29:23 PM No Comments

CRISPR gene editing technology is being used to solve issues concerning how to localize intracellular proteins. Learn more by reading our interview with expert in this field,  Dr. Emma Lundberg

Audio also avaliable

David Shifrin: Welcome to Horizon Discovery. I'm David Shifrin. Today I'm speaking with Dr. Emma Lundberg, Associate Professor at the KTH Royal Institute of Technology and the Science for Life Laboratory. Doctor Lundberg, as we'll discuss in this conversation has a deep interest in looking at protein localization and expression throughout the many tissues of the human body during health and disease, and this comes out in the context of different cell lines in her research. Because of that interest, she's been involved in a number of different projects to ensure that the tools available to researchers for labeling proteins are really well-validated.

Then on the other side of things, Horizon Discovery is building a suite of endogenous pathway tag and reporter cell lines, the idea being that researchers can look at natural levels of protein and promoter activity with fewer artifacts than you'd expect from something like a traditional transfection reagent. The goal is really to help with projects like those that Dr. Lundberg is spearheading in the drive for quality in the antibody arena as well as a number of other projects that researchers might conceive.

With that, Dr. Lundberg, thanks very much for taking the time to speak with me today. How are you?

Emma Lundberg: I'm doing very well, thank you.

Mapping the localization of all human proteins

David Shifrin: Great. I'd like to start kind of big picture with your interest in this whole realm of antibody validation. You were part of a science paper published in early 2015 that mapped protein expression across more than 30 human tissues, and then late 2016 your lab published a paper in the Journal of Proteome Research that was a really interesting paper using endogenously expressed, tagged proteins to validate antibodies that were available through the Human Protein Atlas. Clearly this idea that ensuring that all the tools that researchers use to look at protein expression are very well validated, as I said before, and appropriate for the experiment at hand. Can you tell us a bit more about your interest in this idea and then kind of where all of that came from?

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