CRISPR gene editing technology is being used to solve issues concerning how to localize intracellular proteins. Learn more by reading our interview with expert in this field, Dr. Emma Lundberg
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David Shifrin: Welcome to Horizon Discovery. I'm David Shifrin. Today I'm speaking with Dr. Emma Lundberg, Associate Professor at the KTH Royal Institute of Technology and the Science for Life Laboratory. Doctor Lundberg, as we'll discuss in this conversation has a deep interest in looking at protein localization and expression throughout the many tissues of the human body during health and disease, and this comes out in the context of different cell lines in her research. Because of that interest, she's been involved in a number of different projects to ensure that the tools available to researchers for labeling proteins are really well-validated.
Then on the other side of things, Horizon Discovery is building a suite of endogenous pathway tag and reporter cell lines, the idea being that researchers can look at natural levels of protein and promoter activity with fewer artifacts than you'd expect from something like a traditional transfection reagent. The goal is really to help with projects like those that Dr. Lundberg is spearheading in the drive for quality in the antibody arena as well as a number of other projects that researchers might conceive.
With that, Dr. Lundberg, thanks very much for taking the time to speak with me today. How are you?
Emma Lundberg: I'm doing very well, thank you.
Mapping the localization of all human proteins
David Shifrin: Great. I'd like to start kind of big picture with your interest in this whole realm of antibody validation. You were part of a science paper published in early 2015 that mapped protein expression across more than 30 human tissues, and then late 2016 your lab published a paper in the Journal of Proteome Research that was a really interesting paper using endogenously expressed, tagged proteins to validate antibodies that were available through the Human Protein Atlas. Clearly this idea that ensuring that all the tools that researchers use to look at protein expression are very well validated, as I said before, and appropriate for the experiment at hand. Can you tell us a bit more about your interest in this idea and then kind of where all of that came from?