Horizon Diagnostics Blog

Combatting the challenges of liquid biopsy with cfDNA synthetic plasma Reference Standards

Aug 10, 2018 3:46:08 PM No Comments

Liquid biopsy holds many advantages over solid tumor testing of cancer patients. It is less invasive for the patient, avoiding the need for surgical biopsy, and has improved levels of sensitivity to detect low frequency somatic driver mutations.   

The industry is pushing for liquid biopsy to become the go-to method for the collection of clinical DNA samples for oncology genotyping. This could be at the point of diagnosis - for routine monitoring during treatment to detect the appearance of resistance mutations, which may indicate a change of therapy is required. Alternatively, liquid biopsy could be used for future preventative cancer screening of the general population before disease symptoms appear - to facilitate earlier diagnosis and better treatment outcomes.

Read More Oncology Panels, NGS, cfDNA, Reference Standards, Oncology

Case Study: OGT use Horizon's Reference Standard to validate their SureSeq™ assay technology

May 23, 2018 3:10:58 PM No Comments

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Oxford Gene Technology (OGT), a molecular genetics company who manufacture custom NGS cancer panels and sequencing reagents, used Horizon’s formalin compromised DNA (fcDNA) Reference Standards to validate their SureSeq™ assay technology.

Read More Oncology Panels, NGS, Reference Standards, Oncology

Recent developments in targeting NTRK fusions for cancer therapeutics

Apr 27, 2018 3:24:21 PM No Comments

The NTRK Gene Family

Since the mid-80s, evidence has shown that gene fusions are implicated in cancer. With advances in Next Generation Sequencing (NGS) technology, the impact of gene fusions on driving cancers and how they act as potential therapeutic targets is quickly being revealed.

Read More Oncology Panels, NGS, Reference Standards, RNA, RNA fusions

Why You Should Use Cell Line-Derived Reference Material for NGS Oncology Assays

Apr 12, 2018 2:12:47 PM 1 Comment

 

We all know NGS analysis can be difficult to navigate. Not only is the human genome complex, but technical errors can occur throughout the entire workflow – from sample preparation, through sequencing and during analysis.

Thankfully, these technical errors can be mitigated by using high-quality reference material. Using the best standards for your assay will help both calibrate your NGS measurements and evaluate your diagnostic performance.

With advances in affordable technologies, there is now a multitude of reference types to choose from including patient material, cell line-derived standards and synthetic spike-ins. In this blog, we shine the spotlight on cell line-derived standards and the distinct advantages these have when used for NGS oncology assays.

Read More Oncology Panels, NGS, Reference Standards

Our Top 5 Quality Control (QC) Metrics Every NGS User Should Know

Feb 20, 2018 3:03:09 PM No Comments Comment

We all know how vital quality control is for our samples. A lot of research has gone in to developing useful QC metrics for genomics experiments – primarily due to their high cost. Skipping this step will waste both time and money.

There are 3 main areas where QC can be applied to NGS:

  • On the starting nucleic acids
  • After Library preparation
  • Post-Sequencing
Ultimately the best QC of your NGS experiments is likely to come from the sequence data and, because of this, many labs will run a QC lane first.
Read More NGS

10 Top Tips for Validating your NGS Assay

Feb 6, 2018 11:32:46 AM No Comments Comment

With new advances in technology, we’re now moving more towards large panel Next-Generation Sequencing (NGS) assays and whole exome (WES) and genome sequencing. NGS is gaining popularity thanks to the high-throughput capability and lower cost per sample.

However, validating these complex assays can be tricky, so we’ve compiled some top tips from industry guidelines* to help you...

Read More Oncology Panels, NGS, Reference Standards

The growing impact of liquid biopsies on precision medicine

Oct 11, 2017 10:30:20 AM No Comments Comment

 Liquid biopsies are becoming increasingly popular in cancer research. We take a look at their potential impact in the clinic and how current technologies can help us keep pushing the boundaries of precision medicine. 

Read More NGS, cfDNA, Reference Standards

Detecting RNA Fusions in cancer - the benefits, the challenges and the solution

Aug 25, 2017 4:28:37 PM No Comments Comment

 

RNA-Sequencing (RNA-seq) of oncogenic fusions is becoming increasingly popular in cancer research and diagnosis. Here, we talk about the benefits of using RNA in fusion detection workflows, the challenge of workflow variability and how to be confident of your assay results.
Read More Reference Standards, RNA, RNA fusions

SHERLOCK: The case of the disruptive diagnostic

May 10, 2017 2:07:05 PM No Comments Comment

“SHERLOCK is exciting because it represents a sensitive approach to detect mutations, much like PCR, but it does not require a thermocycler and could work as a bedside test.” - Dr Tilmann Buerckstuemmer, Global Head of Innovation at Horizon Discovery

Read More CRISPR, cfDNA

Top 10 RNA-Seq Publications of All Time

Jan 9, 2017 2:07:51 PM No Comments Comment
"RNA-Seq is the most likely NGS technique to be used by researchers in 2017 due to its versatility"

RNA-seq has become a ubiquitous tool in both biological and medical research. Much of the RNA-seq analysis done is still for differential gene expression from poly-adenylated mRNAs; but the success of RNA-seq can also be seen in the rapid increase in knowledge about biological systems and the large number of distinct variants of the method.  The combination of these factors allow us to go much further than the 'simple' 3’ gene expression microarrays and now opens up the possiblities of: splicing analysis, differential allele expression, variant detection, alternative start/stop, gene fusion detection, RNA editing and eQTL mapping to name but a few...

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