Droplet Digital™ PCR, labelled the third generation of PCR technology, enables the absolute quantitation of target DNA. The platform allows for the enrichment of rare target sequences, the detection of small differences in target concentration and the determination of copy number without the need for a standard curve.
In this study we show the Bio-Rad QX100™ ddPCR™ platform was able to detect mutant alleles (BRAF V600E, BRAF V600K, KRAS G12D, KRAS G12V, EGFR ΔE746-A750) present at frequencies as low as 0.05%. In addition the platform enabled the detection of mutant alleles present as low as 3.5% using DNA extracted from the FFPE Reference Standards.
To read the complete application note, including assay optimisation, validation of Copy Number assays and estimated costs of detecting different allele frequencies on this platform download the complete application note.