The adoption of CRISPR in 2015 was staggering, and keeping up with publications was a challenge. At Horizon, we polled our scientists on what their favorite CRISPR publications of 2015 were and why, and include six highlights below.
Chen et al Cell March 2015
“I’m already convinced that CRISPR screening is going to be completely transformative in drug discovery. But to see a genome wide screen applied in an in vivo context just blew me away. I think the elegance and seeming simplicity of this paper belies the challenge of actually doing this kind of work. Amazing science” – Dr Chris Thorne (Commercial Marketing Manager)
Reddy et al Cell April 2015
“Because it makes the most the most of genome editing and protein targeting technologies to tackle an “impossible problem” – to edit mitochondrial DNA!” – Dr Rodrigo Santos (Cell Line Engineering Scientist)
This paper actually uses TALEN - but we didn't want to leave it out!
Shi et al Nature Biotechnology June 2015
“By targeting CRISPR-Cas9 mutagenesis to exons encoding functional protein domains, Shi et al achieve an extremely robust data set from their screen, and moved forward thinking on best practice for CRISPR knockout library design.” – Dr Jon Moore (CSO)
Parnas et al Cell July 2015
“This is the first work using such technology in primary immune cells. These cells are not as easy to handle as cell lines. Immune cells have evolved to react to every stimulus they get so their manipulation for such big experiments is not as simple. The immunology values from this study can be huge.” – Dr Cristina Ghirelli (Immuno-oncology Team Leader)
Zetsche et al Cell October 2015
“The discovery of a non-CRISPR/Cas enzyme with similar functional attributes – Cpf1. This paper illustrates that this field is only in its infancy and fundamental discoveries are still within reach.” – Dr Tilmann Buerckstuemmer (Cell Line Products R&D Director)
Lackner et al Nature Communications December 2015
“One of the big challenges with conventional endogenous gene tagging is that the tag donor must be designed for each genomic site. In this paper Lackner et al develop a method to efficiently and predictably insert a generic tag at multiple sites. This innovation will have a huge impact on how we work at Horizon” – Dr Benedict Cross (Research Services Team Leader)
What do you think was the most important CRISPR publication in 2015? Drop us a comment below and let us know!
Here’s to another exciting year in genome editing. All the best for 2016.
The Horizon Team
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