Articles, announcements and insights from Horizon Discovery

Top peer reviewed scientific articles using HAP1 cell lines

Mar 13, 2017 10:24:39 AM No Comments

Can I use the HAP1 cell line for my research?

The HAP1 cell line has been applied across a wide range of biological processes, such as DNA damage repair pathway and stress responses, as well as in disease modeling. These selected  articles show the broad applicability of the HAP1 cell line, and provide characterization data to help your research. 

2017

2016

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Probing DNA Damage Response Pathways

Mar 8, 2017 1:02:47 PM No Comments

Sensitivity and resistance to DNA Damage Response Pathways identified with gene-edited cell lines and wildtype controls

The cellular DNA damage response (DDR) is an essential safeguard against cancer. Upon activation, the DDR can limit tumour progression at the early stages by inducing senescence or cell death. When this defence fails tumors are able to develop. However, with time, tumors accumulate more mutations in DNA repair proteins as cancers progress.  The efficiency of DDR plays an essential role in the effectivity of cytotoxic treatments. Currently much research is focussed on identifying the DDR mechanisms involved in cancers and how these dysfunctional processes can be utilized against tumor growth.

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How to Cure Cancer in 3 Easy Steps

Mar 6, 2017 6:10:15 PM No Comments

When I have read articles just like this one early on in my career, I would laugh and categorize it with blogs regarding Bigfoot and the Loch Ness Monster. However, much has changed in the past 10 years. New technologies have been developed and milestones have been reached that should have Cancer a little worried. These 3 steps might be viewed to some as obvious, but I argue that it’s how the researchers have utilized the technology wisely that has made the difference. I have identified some papers that have carved a successful path to Cancer's possible demise.

Read More Cell lines, Gene editing, CRISPR, Oncology Panels, Target identification, Target validation, PDX, Cancer models, Preclinical efficay testing, High Fidelity Models

Spying on proteins - How observation can affect the perception of reality

Mar 2, 2017 2:47:38 PM No Comments

One of the highest ideals in science is to observe natural events in their native context. Doing so is a constant challenge thanks to the Observer Effect, described by Heisenberg and others, where the act of observing or measuring a process alters it. Thus, scientists of all stripes try to get out of the way, attempting to produce the most accurate measurements possible using specific yet unobtrusive tools. Wildlife photographers use long-range lenses to avoid the need to stand directly in front of a herd of water buffalo and thereby affect the animals’ behaviour. Psychologists create tests where the subjects are unaware of the true intent so as to minimize changes in natural responses.

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Large scale gene tagging simplified by a self releasing plasmid

Mar 2, 2017 2:19:09 PM No Comments

Knockout and tagged gene-editing can create cell lines ideal for antibody validation. Using CRISPR CAS technology, Horizon developed a streamlined process to cut production time. Read more in our interview with Lead Scientist Daniel Lackner

Audio also available

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Is there a better way to visualize proteins in cells?

Nov 17, 2016 1:37:26 PM No Comments

 The visualisation of proteins or organelles in cells and other complex biological systems is ‘bread and butter’ stuff for cellular and molecular biologists; it’s performed day-in, day-out in labs across the globe. But this doesn’t mean that the most popular approaches currently used for protein visualisation – dye staining, antibody labelling and fusion protein over-expression systems – are ideal. Almost every scientific technique has its advantages and drawbacks, and the various options currently available for protein visualisation are not exceptions to this rule, so let’s have a look at the pros and cons of each.

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2016 Nobel Prize in Medicine Awarded to Autophagy Pioneer: Professor Yoshinori Ohsumi

Oct 10, 2016 9:42:14 AM No Comments

Recycling has always been a smart idea, and nature has its own processes to ensure that waste is kept to a minimum.  As Professor Ohsumi discovered, autophagy is the cells way of degrading and recycling cellular components, allowing it to adapt to nutritional deficiency or other environmental influences. Professor Yoshinori Ohsumi, honorary professor and leader of the Cell Biology Unit at the Tokyo Institute of Technology, has been studying autophagy for 27 years. This year's Nobel Laureate discovered and elucidated mechanisms underlying autophagy, according to the Press Release from The Nobel Assembly at Karolinska Institutet.

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Optimization of CRISPR Delivery in Cultured Cells & Single Cell Embryos

Jun 13, 2016 10:22:03 AM No Comments

The CRISPR/Cas9 system has been rapidly adapted to practically every model system for its ease to generate and high efficiencies to cleave target DNA. But unlike our experience with Zinc Finger Nucleases, in the human, rat and mouse cell lines we tried successful co-transfection of Cas9 mRNA and sgRNA was cell-line dependent, and often resulted in either very low or no cleavage activities.

However, sequential transfection of cells with Cas9 DNA first, and sgRNA followed 24 hrs later, reliably produced good level of activity, indicating the requirement of Cas9 presence at the time of introduction of sgRNA. Not surprisingly, creation of a cell line stably expressing Cas9 led to consistently high cleavage activities upon transfection of sgRNAs. Transfection of recombinant Cas9 protein pre-complexed with sgRNA (ribonucleoprotein particles, or RNPs) led to efficient cleavage as well.

On the other hand, when Cas9 mRNA and sgRNAs are co-microinjected into single cell embryos, it produces target cleavage as efficiently as RNPs to produce straight KOs and large deletions between two target sites, again raising a question of local concentrations of Cas9 protein and sgRNA.

Below we summarize some of the work we've done optimizing delivery of CRISPR-Cas9, which which can be read in full publication form in Human Gene Therapy here.

Read More Cell lines, Gene editing, Microinjection

Frequently Asked Questions For HAP1 Knockout Cell Lines

Jun 10, 2016 1:29:00 PM No Comments
Here you'll find a complete list of all our most frequently asked questions relating to HAP1 knockout cell lines. If you want to know how they're generated, how they're validated or how to find out if they're right for you - this is a great place to start.
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Frequently asked questions for X-MAN Cell Lines

Jun 10, 2016 1:27:59 PM No Comments

Here you'll find a complete list of our most frequently asked questions relating to X-MAN cell lines. How are they made, how are they validated, and how can you use them? Read on to find out.

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Spying on proteins - How observation can affect the perception of reality

One of the highest ideals in science is to observe natural events in their native context. Doing so...