Articles, announcements and insights from Horizon Discovery

Scientific Reports: Increasing the performance of pooled CRISPR–Cas9 drop-out screening

Nov 18, 2016 10:44:56 AM No Comments

Recently published in a paper on Nature.com in Scientific Reports, Horizon Discovery have conducted a detailed analysis of CRISPR-Cas9 sensitivity (drop-out) screening to come up with a highly improved and optimised platform. In our analysis, we used a custom ultra-complex sgRNA library and capitalised on Horizon’s streamlined screening pipeline to evaluate fundamental aspects of functional genomic screening, including:

  • Side-by-side comparison of the impact of a novel tracrRNA sequence on screen performance
  • Direct analysis of the efficacy of two different sgRNA design algorithms
  • Evaluation of the effect of cell line ploidy on KO rate and screen quality
  • Time-resolved gene drop-out analysis to evaluate the kinetics of CRISPR-Cas9 driven gene knockout
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Using CRISPR for target identification and target validation

Jun 10, 2016 1:51:33 PM No Comments

The discovery of the CRISPR‐Cas system in bacteria has initiated an impressive array of innovations that have enabled the use of the RNA‐guided Cas9 nuclease in functional genomic screens. At Horizon, we have embraced these developments, as they provide new opportunities for drug target identification and validation. The case studies presented in this below highlight how we use this technology to successfully conduct genome wide and focused sgRNA library screens and to verify whether specific genes are required for the survival and/or proliferation of cancer cell lines.

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A Guide To CRISPR Screening

Jun 8, 2016 5:09:13 PM No Comments

Thanks to next generation sequencing (NGS), we are starting to understand the mutational changes that occur across the board in the cancer genome. With this knowledge comes potential – novel mutated genes and the proteins that they encode are candidates for prognostic markers and/or new drug targets.

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Seven must-read CRISPR screening papers

Jun 8, 2016 5:05:13 PM No Comments

It’s hard to keep up with the rapidly expanding world of CRISPR, and it’s starting to feel like CRISPR screens are being published every week, taking the technique from the cutting edge to the mainstream.

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