Gene modulation assays can be tricky, even for the most experienced researcher, so we have compiled some tips to help you.
Our team of PhD trained scientists have put together their top 5 resources they share with customers doing RNAi experiments. These were written with all the most common customer questions in mind. This is a must-have resource, truly worthy of a bookmark on your favorites bar!
Which controls do you need?
The path to positive results begins with setting up your RNAi experiment with the right controls. In this resource, "Ensure success with appropriate controls in your RNAi experiments", we have outlined the key controls to include and why.
Steps to guaranteed silencing in difficult-to-transfect cells
Researchers working with cells that are difficult to transfect can regularly find success with Dharmacon™ Accell™ siRNAs. Our "Guaranteed silencing with Accell siRNA in 3 easy steps" article covers the key steps that will ensure guaranteed gene knockdown when using these siRNAs.
Troubleshooting RNAi knockdown
When your RNAi experiment doesn’t seem to be working, it can be tough to know where to begin troubleshooting. In this article, "Top three tips for troubleshooting your RNAi experiment" we go through some common questions we ask at each step of the process, so you can get your assay back on track.
All the features on our siRNA custom design tool, siDESIGN
For customers designing their own siRNA, this article "Variant-specific knockdown by siRNA" shows how to fine-tune our siDESIGN web tool to make the best siRNA candidates for knockdown experiments.
Top considerations when using Western blotting
Protein absence, presence or abundance is a pretty common way to analyze the success of your RNAi experiment. In the "Is your antibody binding the right target?" article we go through some important considerations for choosing your protein detection antibody, so you can distinguish gene expression and knockdown with confidence.