HAP1 cell lines are a popular choice to validate a range of research experiments, but if you’ve never used them before, you want to be sure they are right for you.
CRISPR-Cas9 is a very versatile tool to discover more about your pathway or gene of interest. After all that hard work editing your cell line, you want to have the confidence to rely on your new research model. So, how do you verify your cell line is what you expect it to be?
There are a couple of areas to consider:
- Cell line clonality - a mixed population may obscure the effects of your desired gene edits
- Is the targeted gene edit affecting the cell line phenotype, or is this due to off-target effects?
Here are a few ways to add supporting data to validate your gene-engineering projects...
CRISPR-edited Cell Lines are a great tool to validate your antibodies before you start your experiments. They ensure you are using high quality reagents, so you can be confident in your results. We explain how you can use Cell Lines for validation, the challenges to be aware of and how we can help you overcome them.
A major study has been undertaken to gain a better understanding of thousands of mutations in the BRCA1 gene – a key gene in breast and ovarian cancers.
Published in Nature this month, the study by the Department of Genome Sciences at the University of Washington School of Medicine, set out to analyze nearly 4,000 variants in 13 exons of BRCA1 that are “of unknown significance”. These are variants that are not currently known to cause cancer, but theoretically could.
With over 25,000 individual Cell Lines in our new Express Catalog, we want to give you a helping hand with finding the ideal Cell Line Model for your research.
Using our new downloadable catalog, you can now search, filter and browse through our online cell model offering. There are two main collections available in our catalog:
- Knockout Cell Models
- Cancer-relevant Cell Lines
To help you find the right cell line for your research, here's some bite-size information on the data available to you...
CRISPR technology now allows genes and molecular pathways to be examined with greater definition. We look at how knockout cell lines, either together with gene rescue and replication of disease mutations or as an independent cell model, can be used to validate your research and extend your findings.
There is a significant challenge in translating the wealth of genetic information now available to the role of genes, to understand basic biology, as well as linking to the role of mutations for understanding disease pathogenesis.
Knockout (KO) cell lines are excellent model systems to do this. A key benefit of cell lines is the ability to use gene-editing to construct isogenic cell line pairs - where a mutant model can be interrogated alongside a wildtype control.
Here’s five great examples of how to get the most out of your research using KO Cell Lines:
Due to obvious ethical considerations, cancer research conducted in humans is restricted to observational and analytical studies, with therapy focused clinical trials being the one exception to this rule. Preclinical mouse tumour models therefore provide a critical intermediate experimental model system tying together more basic in vitro research with studies in humans, thereby providing bench-to-bedside translational oncology research.
Optogenetics, a neuromodulation method that is employed to control and monitor the activities of individual neurons in living tissue and was first developed by researchers Edward Boyden and Karl Deisseroth in 20051, is now considered as being one of the main pillars of neuroscience research.
In 2010, the journal Nature Methods choose optogenetics as the ‘Method of the Year’2 across all fields of science and engineering. In the same year the academic research journal Science highlighted it in the article ‘Breakthroughs of the Decade’3. The basis of this technique is to genetically modify neurons to express light-sensitive ion channels such as excitatory channelrhodopsin or inhibitory halorhodospin and then use light to control the on/off status of neuronal excitation. Great progress has been made with this technology since its invention and today even conscious free-moving animal models can be manipulated and observed in real-time.
Have you been overwhelmed by the number of CRISPR articles published in 2017? PubMed alone has cited over 3,000 CRISPR publications in 2017! We wanted to save you from having to sieve through the databases by asking our experts to select what they thought were the most important CRISPR publications from last year.
For the first time, Human Knockout cell lines are readily available for scalable reverse genetic screening.
We speak to Horizon's Head of Innovation, Dr Tilmann Bürckstümmer about the application of reverse genetic screening using a combination of new technologies.
Gapp et al. (2016) in Molecular Systems Biology