CRISPR-Cas9 is a very versatile tool to discover more about your pathway or gene of interest. After all that hard work editing your cell line, you want to have the confidence to rely on your new research model. So, how do you verify your cell line is what you expect it to be?
There are a couple of areas to consider:
- Cell line clonality - a mixed population may obscure the effects of your desired gene edits
- Is the targeted gene edit affecting the cell line phenotype, or is this due to off-target effects?
Here are a few ways to add supporting data to validate your gene-engineering projects...